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目的:通过观察止消通脉宁含药血清对转化生长因子-β1(TGF-β1)诱导人肾小管上皮细胞(HK-2)增殖的影响,探讨治疗糖尿病肾病有效中药复方止消通脉宁在防治肾间质纤维化方面的作用。方法:将HK-2细胞用含10%胎牛血清的DMEM/F12(1∶1)培养基培养;实验分为6组:空白对照组、单纯TGF-β1诱导组(TGF-β110ng/mL)、空白血清对照组(TGF-β110 ng/mL+10%空白血清)、干预1组(TGF-β110 ng/mL+10%低剂量止消通脉宁含药血清)、干预2组(TGF-β110 ng/mL+10%中剂量止消通脉宁含药血清)、干预3组(TGF-β110 ng/mL+10%高剂量止消通脉宁含药血清)。倒置显微镜下观察各组细胞形态,MTT法检测细胞增殖。结果:TGF-β110 ng/mL能显著诱导人肾小管上皮细胞增殖,与空白对照组相比有显著性差异(P<0.05),且其作用随处理时间的延长而增强,但和止消通脉宁含药血清共同作用后,其促细胞增殖作用受到一定抑制(P<0.05)。结论:止消通脉宁能抑制HK-2细胞增殖,在一定程度上具有防治肾间质纤维化的作用。
OBJECTIVE: To observe the effect of Zhixiaotongmaining-containing serum on the proliferation of human renal tubular epithelial cells (HK-2) induced by transforming growth factor-β1 (TGF-β1) and to study the effect of Zhuxintongmaining In the prevention and treatment of renal interstitial fibrosis role. Methods: HK-2 cells were cultured in DMEM / F12 (1: 1) medium containing 10% fetal bovine serum. The experiment was divided into 6 groups: blank control group, TGF- , TGF-β110 ng / mL + 10% blank serum) and intervention group 1 (TGF-β110 ng / mL + 10% β110 ng / mL + 10% of the medium-dose anti-Tongmai Ning containing serum), intervention group 3 (TGF-β110 ng / mL + 10% high dose Zhi Tong Tong Mai Ning serum). The morphology of each group was observed under inverted microscope, and cell proliferation was detected by MTT assay. Results: TGF-β110 ng / mL significantly induced the proliferation of human renal tubular epithelial cells, which was significantly different from that of the blank control group (P <0.05), and its effect increased with the prolongation of treatment time. However, After the drug-containing serum of Mai-ning cooperated, the cell proliferation was inhibited to a certain extent (P <0.05). Conclusion: Zhuxiaotongmai can inhibit the proliferation of HK-2 cells to a certain extent, with the prevention and treatment of renal interstitial fibrosis.