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目的探讨食管鳞状细胞癌及癌前病变组织p16基因CpG岛的甲基化情况,及其在食管癌发生和早期诊断中的价值。方法采用MSP方法,对食管癌前病变不同阶段、鳞状细胞原位癌和浸润癌的病变组织进行了甲基化检测,并与其相应的正常组织和慢性食管炎组织的甲基化情况进行对比分析。结果轻、中、重度不典型增生、鳞状细胞原位癌和浸润癌的甲基化频率分别为22.73%(5/22)、46.15%(6/13)、77.78%(7/9)、78.57%(11/14)和64.86%(24/37)。67例正常对照组织中3例(4.48%)p16基因甲基化,与病变组织相比,差异有统计学意义,P=0.000。10例慢性食管炎组织中1例(10%)p16基因甲基化。结论p16基因甲基化可能是食管癌发生的最早期事件之一。
Objective To investigate the methylation of p16 gene CpG island in esophageal squamous cell carcinoma and precancerous lesions and its value in the diagnosis and early diagnosis of esophageal cancer. Methods MSP was used to detect the methylation of lesions in squamous cell carcinoma of the squamous cell carcinoma and invasive squamous cell carcinoma in different stages of esophageal precancerous lesions. The methylation status was compared with the corresponding normal tissues and chronic esophagitis analysis. Results The frequencies of methylation in squamous cell carcinoma in situ and invasive squamous cell carcinoma were 22.73% (5/22), 46.15% (6/13) and 77.78% (7/9), respectively. 78.57% (11/14) and 64.86% (24/37) respectively. The methylation of p16 gene in 3 cases (4.48%) of 67 normal control tissues was statistically different from that in the diseased tissues (P = 0.000). One case (10%) of the 10 patients with chronic esophagitis had p16 gene methylation Based on. Conclusion The methylation of p16 gene may be one of the earliest events of esophageal cancer.