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目的 探讨成年Wister大鼠在坐骨神经切断后GAP-43于相应脊髓节段前角运动神经元内的表达变化。方法 选取健康成年雄性Wister大鼠60只,随机分为坐骨神经切断组和对照组,分别于术后1、2、4、8、12、16周处死后取其L4～L6脊髓,利用免疫组织化学技术检测GAP-43在相应脊髓节段中的表达变化,并利用影像分析系统进行统计学分析。结果 在对照组中GAP-43表达呈阴性,随着时间的变化无明显改变。坐骨神经切断组与对照组在不同时相中的变化:1周时对照组中胞体内GAP-43有明显表达,2周达到高峰,4～8周时呈下调趋势,9～16周时GAP-43在神经元中仍有表达。结论 坐骨神经切断可导致成年大鼠相应脊髓阶段中前角运动神经元GAP-43表达明显增加,表明周围神经损伤后,神经元的再生能力增强,但并不能长时间持续。
Objective To investigate the expression of GAP-43 in the anterior horn of spinal cord in adult Wister rats following sciatic nerve transection. Methods Sixty healthy adult male Wistar rats were randomly divided into sciatic nerve transection group and control group. L4 ~ L6 spinal cord were harvested at 1, 2, 4, 8, 12 and 16 weeks after operation respectively. Immunohistochemistry The expression of GAP-43 in the corresponding spinal cord segments was detected by the technique and analyzed by the image analysis system. Results In the control group, GAP-43 expression was negative, with no significant change over time. The changes of GAP-43 in the sciatic nerve cut-off group and the control group in different phases at 1 week were significantly increased in the control group, peaked at 2 weeks, and decreased at 4 to 8 weeks. At 9 to 16 weeks, GAP- 43 is still expressed in neurons. Conclusion Sciatic nerve transection can cause the expression of GAP-43 in anterior horn motor neurons in the corresponding spinal cord phase of adult rats significantly increased, indicating that the neuronal regeneration ability is enhanced after peripheral nerve injury, but not for a long time.