论文部分内容阅读
目的 研究成釉细胞瘤肿瘤细胞巢周边及中心区细胞增殖和分化的特点。方法 选取43例成釉细胞瘤 ,包括滤泡型 15例、丛状型 2 0例和棘皮瘤型 8例。应用免疫组化SP方法检测标本组织中增殖细胞核抗原 (proliferatingcellnuclearantigen ,PCNA)、Ki 6 7、CK10&13和CK19蛋白的表达。结果 PCNA在肿瘤细胞巢周边区的增殖指数 (5 12± 2 76 ) %显著高于中心区 (1 36± 1 0 2 ) % ,P <0 0 0 1;Ki 6 7在肿瘤细胞巢周边区的增殖指数 (3 6 3± 1 80 ) %显著高于中心区 (1 2 6± 0 96 ) % ,P <0 0 0 1;PCNA和Ki 6 7的增殖指数有显著相关关系 (P <0 0 1) ;CK10&13和CK19在成釉细胞瘤中心区细胞表达显著高于周边区 (P <0 0 1)。结论 成釉细胞瘤肿瘤细胞巢周边区为增殖细胞区 ,周边区和中心区细胞的分化程度不同。
Objective To study the characteristics of proliferation and differentiation of cells in the periphery and center of ameloblastoma nests. Methods 43 cases of ameloblastoma were selected, including 15 cases of follicular type, 20 cases of plexiform type and 8 cases of type of acromioccosis. Immunohistochemical SP method was used to detect the expression of proliferating cell nuclear antigen (PCNA), Ki 6 7, CK 10 & 13 and CK 19 in the specimens. Results The proliferation index (PC12) of PCNA in the peripheral region of tumor nests was significantly higher than that in the central region (136 ± 102%), P <0.01 or Ki67 (3 6 3 ± 1 80)% was significantly higher than that in the central area (1 2 6 ± 0 96)%, P 0 01 0, and there was a significant correlation between the proliferative index of PCNA and Ki 6 7 The expression of CK10 & 13 and CK19 in the central ameloblastoma cells was significantly higher than that in the peripheral area (P <0.01). Conclusion Ameloblastoma tumor cell nests surrounding the proliferation of cell areas, peripheral and central cell differentiation degrees.