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我们根据Ｍｅｙｅｒ等研究结果，参照Ｍａｒｃｈｅｔｔｉ等的方法并加以改进，分别采用单纯酶与复合酶消化法分离成猪胰岛，并对两种方法做了比较，现将结果报道如下。一、材料与方法１．实验材料 （１）主要试剂：①胶原酶Ⅳ型（美国ｓｉｇｍａ公司生产）；②弹性蛋白酶（美
According to the results of Meyer et al., And according to the method of Marchetti et al., We improved the method and separated it into pig islet by simple enzymatic and compound enzymatic digestion respectively. The results are reported as follows. First, materials and methods 1. Experimental materials (1) The main reagents: ① collagenase type Ⅳ (Sigma company produced in the United States); ② elastase