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目的:研究白细胞介素-1受体相关激酶-2(IRAK-2)反义寡核苷酸对白细胞介素-1(IL-1)和肿瘤坏死因子(TNF)诱导前列环素(PGI_2)合成的不同影响。方法:IRAK-2反义寡核苷酸(IRAK-2 ODN)转染脐静脉内皮细胞以阻断IRAK-2的表达,以IL-1及TNF刺激细胞后,用竞争ELISA方法检测PGI_2的合成。结果:IRAK-2 ODN可显著降低IL-1诱导的PGI_2合成,此效应强度存在时间和浓度依赖性,但IRAK-2ODN对TNF诱导的PGI_2合成无影响。结论:IRAK-2 ODN对IL-1和TNF诱导PGI_2合成有不同影响。IRAK-2在IL-1诱导的PGI_2合成中起重要作用。
Objective: To investigate the effects of interleukin-1 receptor associated kinase-2 (IRAK-2) antisense oligonucleotide on interleukin-1 (IL-1) and tumor necrosis factor (TNF) induced prostacyclin (PGI_2) Different effects of synthesis. METHODS: IRAK-2 ODN was transfected into human umbilical vein endothelial cells to block the expression of IRAK-2. IL-1 and TNF-stimulated cells were used to detect the synthesis of PGI_2 by competitive ELISA . Results: IRAK-2 ODN significantly reduced IL-1-induced PGI 2 synthesis in a time and concentration-dependent manner. However, IRAK-2 ODN had no effect on TNF-induced PGI 2 synthesis. Conclusion: IRAK-2 ODN has different effects on IL-1 and TNF-induced PGI2 synthesis. IRAK-2 plays an important role in IL-1-induced PGI2 synthesis.