对13个大白菜品种(品系)进行了游离小孢子培养,从12个品种中诱导出胚,最高诱导效率为每花蕾平均产胚178个。对培养小孢子的发育过程进行了系统观察,结果表明,接种后小孢子先于35℃高温下处理一天,对小孢子发育成胚十分必要。本结果为芸薹属作物花药培养和游离小孢子培养提供了细胞形态学方面的依据。 Thirteen Chinese cabbage cultivars (lines) were isolated from microspore culture in vitro and embryos were induced from 12 cultivars. The highest induction efficiency was 178 embryos per flower bud. The microspore development process was systematically observed. The results showed that the microspores inoculated pre-incubated at 35 ℃ for one day, the microspore embryo development is necessary. The results provided basis for cell morphology in anther culture and microspore culture of Brassica crops.