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目的 :探讨新的人凋亡相关基因产物TFAR19蛋白对羟基喜树碱诱导人 772 1肝癌细胞凋亡的增敏作用。方法 :将不同浓度的rhTFAR19蛋白单独或与羟基喜树碱 (hydroxycamptothecin ,HCPT)同时加入处于指数生长期的 772 1肝癌细胞中共同培养 ,通过透射电镜、DNA片段化分析、PI及AnnexinV标记进行流式细胞仪分析 ,观察细胞的形态学和细胞凋亡的变化。结果 :不同质量浓度的rhTFAR19蛋白单独作用于 772 1肝癌细胞未见明显凋亡 ,但同时加 5mg·L-1的羟基喜树碱后 ,各质量浓度组均观察到细胞凋亡 ,并且显示了明确的量效关系 :5mg·L-1的HCPT加 5mg·L-1rhTFAR19蛋白的细胞凋亡率为 2 9.5 8% ,而加 2 0mg·L-1rhTFAR19蛋白的细胞凋亡率为6 3 .77%。结论 :rhTFAR19蛋白对HCPT诱导的细胞凋亡有明显的剂量依赖性促进作用 ,临床用HCPT行局部化疗时 ,若加rhTFAR19蛋白 ,可能会在不增加化疗药物毒性的情况下 ,提高化疗效果
Objective :To investigate the sensitization of a novel human apoptosis-related gene product TFAR19 protein on hydroxycamptothecin-induced apoptosis of human 772 1 hepatocellular carcinoma cells. METHODS: Different concentrations of rhTFAR19 protein were incubated with hydroxycamptothecin (HCPT) alone or in exponentially growing 772 1 hepatocellular carcinoma cells. Transmission electron microscopy, DNA fragmentation analysis, PI, and Annexin V labeling were performed. Cytometry analysis was performed to observe the changes in cell morphology and apoptosis. RESULTS: Different concentrations of rhTFAR19 protein alone had no obvious apoptosis in 772 1 liver cancer cells, but after adding 5 mg·L-1 of hydroxycamptothecin, apoptosis was observed in each concentration group and it was shown that The specific dose-response relationship was: the apoptosis rate of 5 mg·L-1 HCPT plus 5 mg·L-1rhTFAR19 protein was 29.58%, while the apoptosis rate of 20 mg·L-1rhTFAR19 protein was 63.77. %. Conclusion : rhTFAR19 protein has a significant dose-dependent effect on apoptosis induced by HCPT. In the clinical treatment of HCPT with local chemotherapy, adding rhTFAR19 protein may increase chemotherapy effect without increasing the toxicity of chemotherapeutic drugs.