论文部分内容阅读
目的 探讨组胺H2 受体阻滞剂对免疫介导小鼠再生障碍性贫血 (再障 )的发生有无阻断作用。方法 通过给全身亚致死量照射的BALB/c小鼠转输H 2相同、MLS不同的DBA/ 2小鼠胸腺、淋巴结混合细胞诱导小鼠再障发生 ,实验组每天腹腔注射 1次甲氰咪胍 ,共 8次 ,于照射后第 14天 ,观察股骨骨髓增生程度。结果 腹腔注射 0 .5 0 ,1.0 0 ,2 .0 0mg·(10g体重 ) -1·d-1甲氰咪胍的小鼠的股骨平均骨髓造血组织容量分别为 (4 6 .5 8± 2 0 .41) %、(5 1.88± 2 4.94) %和 (5 9.12± 32 .48) % ,显著高于再障组的 (19.99± 10 .98) % (P <0 .0 1) ,而与单纯照射组 (5 3 .81± 31.72 ) %相比 ,差异无显著性 (P >0 .0 5 )。结论 组胺H2 受体阻滞剂能阻断半相合淋巴细胞诱导经亚致死量照射的小鼠的再障的发生。
Objective To investigate whether histamine H2 receptor blockade can block the immune-mediated aplastic anemia (AA) in mice. Methods The BALB / c mice that were irradiated with sublethal dose of the whole body were transplanted with the same H 2. The mixed mice of thymus and lymph node with different MLS of DBA / 2 mice induced the aplastic anemia. The mice in the experimental group were injected intraperitoneally with cimetidine Guanidine for a total of 8 times. On the 14th day after irradiation, the degree of femoral bone marrow hyperplasia was observed. Results The average femur hematopoietic tissue volume of mice given intraperitoneal injection of 0.5, 1.0, 2.0 mg · (10g body weight) -1 · d-1 cimetidine was (46.58 ± 2) (P <0.01), P <0.01), (5 1.88 ± 2 4.94)% and (5 9.12 ± 32.48)%, respectively, which were significantly higher than those in the AA group (19.99 ± 10.98% There was no significant difference between the two groups (P <0.05) compared with the control group (53.81 ± 31.72)% (P> 0.05). Conclusion Histamine H2 blockers can block the occurrence of aplastic anemia induced by semi-coincident lymphocytes induced by sublethal radiation in mice.