乌梢蛇(Zaocys dhumnades Cantor)药用历史悠久,临床用量大,商品药材中一般又去皮,故时常有品种混乱,出现伪假现象。常见的伪品有红点锦蛇(Elaphe rufodorsata Ca—ntor)和黑眉锦蛇(E.taeniura Cope)等。这3种蛇同属游蛇科动物,去皮药材形体相似。为此,我们采用聚丙烯酰胺凝胶电泳进行鉴别,收到了较好的效果。 1.试剂配制和凝胶制备试剂和凝胶常 Zaocys dhumnades Cantor has a long history of medicinal use, and has a large amount of clinical use. It is generally peeled off in commercial medicinal materials. Therefore, there are often varieties that are chaotic and have pseudo-false phenomena. Common artifacts include the Elaphe rufodorsata Ca-ntor and the E. taeniura Cope. These three kinds of snakes belong to the same family of genus Muscovy, and their peeling herbs are similar in shape. For this reason, we used polyacrylamide gel electrophoresis to identify and received good results. 1. Reagent preparation and gel preparation Reagents and gels