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目的：研究糖皮质激素（ＧＣＯＲ）诱导小鼠记忆功能降低及其作用机理。方法：采用Ｆｕｒａ２／ＡＭ 钙离子荧光指示剂和双波长荧光分光光度计测定细胞内［Ｃａ２＋ ］ｉ 浓度；用透射电镜和流式细胞仪分别检测细胞凋亡（ＰＣＤ） 。结果：ＧＣＯＲ 可分别诱导成年及老龄小鼠机体免疫功能降低，胸腺及脾脏脏器指数减小，老龄动物记忆功能降低，胸腺、海马细胞内Ｃａ２＋ 超载，海马神细胞ＰＣＤ细胞数增加。体外试验结果亦表明，ＧＣＯＲ 可直接诱导Ｔ 淋巴细胞和海马神经细胞［Ｃａ２ ＋］ｉ 超载，细胞凋亡。结论：糖皮质激素可诱导小鼠记忆功能降低。细胞内［Ｃａ２＋ ］ｉ 超载可能是引起小鼠免疫功能降低、智力水平下降、衰老和细胞凋亡的主要原因之一。
Objective: To investigate the mechanism of GCOR-induced memory loss in mice and its mechanism. Methods: The concentration of [Ca2 +] i in cells was determined by Fura2 / AM calcium ion fluorescence indicator and dual-wavelength fluorescence spectrophotometer. Cell apoptosis (PCD) was detected by transmission electron microscopy and flow cytometry. Results: GCOR could induce the decrease of immune function, thymus and spleen organ index, the decrease of memory function in aging animals, the overloading of Ca2 + in thymus and hippocampus and the increase of PCD cells in hippocampal neurons in adult and aged mice, respectively. In vitro test results also show that, GCOR can directly induce T lymphocytes and hippocampal neuronal [Ca2 +] i overload, apoptosis. Conclusion: Glucocorticoid can reduce the memory of mice. Overloading of intracellular [Ca2 +] i may be one of the main causes of decreased immune function, mental retardation, aging and apoptosis in mice.