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利用免疫细胞化学双标技术，研究了大鼠后脚掌注入ｆｏｒｍａｌｉｎ时脊髓ｃ－Ｆｏｓ蛋白和ＮＭＤＡ受体在脊髓灰质不同层次神经元的分布。结果表明，脊髓背角Ⅰ－Ⅱ层、Ⅴ－Ⅵ层、和Ｘ层中的Ｆｏｓ样免疫反应阳性神经元中分别有２８．６％，５８．１％和６４．８％呈现ＮＭＤＡ受体样免疫反应阳性。此结果提示长时间的外周伤害性刺激作用时，脊髓不同层次的伤害性感受神经元可能由于ＮＭＤＡ受体分布的不同而在持续痛刺激过程中起着不同的作用。
The immunocytochemical double labeling technique was used to study the distribution of c-Fos protein and NMDA receptor in different levels of neurons in the spinal cord after formalin injection. The results showed that 28.6%, 58.1% and 64.8% of the Fos-like immunoreactive neurons in the spinal cord dorsal horn Ⅰ-Ⅱ, Ⅴ-Ⅵ and in the X-layer showed NMDA receptor-like Immunoreactive. The results suggest that long-term peripheral noxious stimulation may have different effects on the nociceptive neurons in different levels of the spinal cord due to the different distribution of NMDA receptors.