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目的:观察柴胡解毒汤对实验性酒精性肝纤维化复合模型大鼠Col-Ⅳ、HA血清含量的影响。方法:通过采用酒、猪油混合灌胃诱导大鼠实验性酒精性肝纤维化复合模型,并分为柴胡解毒汤干预Ⅰ组(简称干预Ⅰ组)、柴胡解毒汤兼酒精高脂饮食组(简称干预Ⅱ组)、酒精兼高脂饮食组(简称模型组)及空白对照组予以灌胃,最终取大鼠血清进行检测。称量大鼠体重,并用ELISA Kit法检测透明质酸(HA)和Ⅳ型胶原(Col-Ⅳ)的含量。结果:12周后,造模各组大鼠毛发灰暗,杂乱,消瘦,活动度减弱,大便溏稀。血清Col-Ⅳ、HA较空白对照组均有显著升高(P<0.01),与模型组比较,干预Ⅰ组和干预Ⅱ组均下降明显(P<0.01)。干预Ⅰ组、干预Ⅱ组、模型组、空白对照组HA含量依次为219.17±41.64、184.89±22.83、249.32±34.60、123.48±25.85。Col-Ⅳ含量依次为9.59±1.81、12.57±1.41、19.71±6.04、7.62±5.36。结论:柴胡解毒汤可能通过抑制Col-Ⅳ、HA的表达水平,对大鼠实验性酒精性肝纤维化复合模型具有一定的干预作用。
Objective: To observe the effect of Chaihu Jiedu Decoction on the serum levels of Col-Ⅳ and HA in experimental alcoholic liver fibrosis rat model. Methods: The experimental alcoholic hepatic fibrosis model was induced by mixed gavage of wine and lard, and divided into group Ⅰ (intervention group Ⅰ), Bupleurum detoxification decoction and alcohol high fat diet Group Ⅱ (intervention group Ⅱ), alcohol and high-fat diet group (model group) and blank control group were given gavage, and finally take the rat serum for testing. The body weight of the rats was weighed and the contents of HA and Col-Ⅳ were detected by ELISA kit. Results: After 12 weeks, all the rats in the model group had gray hair, messy, thinner and weakened activity, stool thinning. Serum Col-Ⅳ, HA were significantly increased compared with the control group (P <0.01), compared with the model group, the intervention group Ⅰ and intervention group Ⅱ decreased significantly (P <0.01). The content of HA in intervention group I, intervention group II, model group and blank control group were 219.17 ± 41.64,184.89 ± 22.83,249.32 ± 34.60,123.48 ± 25.85. The contents of Col-Ⅳ were 9.59 ± 1.81, 12.57 ± 1.41, 19.71 ± 6.04 and 7.62 ± 5.36, respectively. Conclusion: Chaihu Jiedu Decoction may play an intervention role in the experimental model of alcoholic liver fibrosis in rats by inhibiting the expression of Col-Ⅳ and HA.