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研究黄精(Polygonatum sibiricum,PS)对CCl4诱导的大鼠肝损伤的保护作用及其抗氧化机制.采用一次性腹腔注射50％四氯化碳油溶液建立大鼠急性肝损伤模型,用黄精水提物(PSAE)灌胃治疗,水飞蓟素作为阳性对照药物,连续7天.PSAE显著降低了血清丙氨酸转氨酶、天冬氨酸转氨酶和碱性磷酸酶(AST、ALT和ALP)的水平(P＜0.05),增加了肝组织谷胱甘肽(GSH)和超氧化物歧化酶(T-SOD)的水平(P＜0.05),降低了肝组织中丙二醛的活性(P＜0.05),并显著降低了肝细胞中的活性氧水平(P＜0.05).在肝组织中,核因子类红细胞2相关因子2(Nrf2),NAD(P)H醌脱氢酶1(NQO-1),血红素加氧酶1(HO-1)mRNA的表达水平显著升高,p53 mRNA表达显著降低(P＜0.05),Bcl-2(P＜0.05)和Bcl-xL mRNA的表达升高,HO-1蛋白表达升高,Keap-1 mRNA表达无明显差异(P＞0.05).因此,黄精对CC14诱导的急性肝损伤有较好的保护作用,其机制与有效调节Nrf2-Keap1-ARE通路相关基因和蛋白质的表达、抑制p53途径介导的肝细胞凋亡有关.“,”In the present study,we aimed to investigate the hepatoprotective effect of Chinese herbal medicine Polygonatum sibiricum(PS).In this study,a rat acute liver injury(ALI)model was established by a single intraperitoneal injection of 50％CCl4oil solution,and the rats were treated intragastrically with Polygonatum sibiricum aqueous extract(PSAE).The results showed that PSAE significantly decreased the serum levels of ALT,AST and ALP,increased the activities of glutathione(GSH)and superoxide dismutase(SOD),decreased malondialdehyde(MDA)activity in hepatic tissue,and decreased the reactive oxygen species(ROS)level in hepatocytes.The expressions of Nrf2,NQO-1,HO-1,Bcl-2,Bcl-xL mRNA,and HO-1 proteins were elevated,and the expression of p53 mRNA was decreased.In conclusion,PSAE exerted a powerful protective action against CCl4-induced ALI in rats via effectively regulating the expressions of Nrf2-Keap 1-ARE related genes and proteins,and inhibiting hepatocyte apoptosis.These outcomes provided evidence that PS had apparent hepatoprotective effect.