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目的:冰片和薄荷脑是清咽滴丸中主要挥发性有效成分。本研究拟建立大鼠ig清咽滴丸后血浆中龙脑和薄荷脑同时测定的气相色谱分析方法,阐明清咽滴丸中龙脑、薄荷脑大鼠体内药代动力学特征。方法:血浆样品采用内标法并经液-液萃取处理,同时采用氢火焰离子化检测器(FID)检测龙脑和薄荷脑大鼠体内的血药浓度。结果:本法可同时测定龙脑、薄荷脑的大鼠血浆浓度,并采用DAS(Version1.0)软件拟合药代动力学参数,结果表明龙脑和薄荷脑均符合开放性一室模型。龙脑在2.5～50.0ng/μL(r=0.9963),薄荷脑在8.7～62.2ng/μL(r=0.9994)均具有良好的线性关系,最低定量限分别为2.4、5.0ng/μL。结论:建立了灵敏、简便、准确的气相色谱法,该方法适用于清咽滴丸中冰片和薄荷脑体内药代动力学研究,为清咽滴丸制剂的临床合理用药提供了理论依据。
OBJECTIVE: Borneol and menthol are the main volatile active ingredients in Qingyan Drop Pills. In this study, we intend to establish a gas chromatographic method for the simultaneous determination of borneol and menthol in plasma of ig qingyan dripping pills, and to clarify the pharmacokinetics of borneol and menthol in rats. Methods: Plasma samples were processed by liquid-liquid extraction with internal standard method and plasma concentrations of borneol and menthol in rats by hydrogen flame ionization detector (FID). Results: The present method can be used to determine the concentration of rat brain in farnesene and menthol. The pharmacokinetic parameters were fitted by DAS (Version1.0) software. The results showed that both borneol and menthol fit the open one-compartment model. There was a good linear relationship between borneol at 2.5 ~ 50.0 ng / μL (r = 0.9963) and menthol at 8.7 ~ 62.2 ng / μL (r = 0.9994). The minimum limit of quantification was 2.4 and 5.0 ng / μL, respectively. Conclusion: A sensitive, simple and accurate gas chromatography method was established. This method is suitable for the study of pharmacokinetics of borneol and menthol in Qingyan Dropping Pills, and provides a theoretical basis for the clinical rational drug use of Qingyan Drop Pill.