论文部分内容阅读
目的探讨新型异黄酮化合物(T1)对雄性SD大鼠由辐射引起的造血系统损伤的防护作用。方法将60只SD大鼠分为正常对照组、辐射组、T1预防组和T1治疗组,每组15只。4.5 Gy60Coγ射线一次性全身照射大鼠(正常对照组除外),T1预防组和T1治疗组大鼠分别在照射前和照射后按剂量为10 mg/kg腹腔注射T1溶液,辐射组在受照后不给予任何处理。动态观察各组大鼠受照前后体质量和外周血WBC 4周内的变化,比较照后1周和4周各组骨髓的病理学变化,并利用比色法测量照后1周各组肝脏组织抗氧化酶的活性。结果受照大鼠的体质量与正常对照组相比,体质量明显减轻(P<0.05);在照后第1、4、5天,T1预防组和T1治疗组的WBC明显高于辐射组(P<0.05),在T1预防组和T1治疗组间差异不明显,从第6天开始,WBC进入恢复期,除正常对照组外,各组WBC无明显差异(P>0.05);受照后1周,辐射组骨髓造血细胞明显减少伴脂肪组织显著增生,T1预防组和T1治疗组损伤相对较轻;在T1预防组,肝脏匀浆CAT、GSH-Px活力较辐射组明显升高,MDA水平明显降低(4.06±0.19vs5.61±0.46,P<0.05)。结论 T1可以减缓照后WBC的下降速度,使WBC的回升时间提前,并对辐射造成的骨髓损伤具有一定的防护作用,其机制可能与消除体内过量有害自由基有关。
Objective To investigate the protective effect of novel isoflavone (T1) on hematopoietic system injury induced by radiation in male SD rats. Methods Sixty SD rats were divided into normal control group, radiation group, T1 prevention group and T1 treatment group, with 15 rats in each group. Rats in the T1 prevention group and the T1 treatment group were injected with T1 solution at a dose of 10 mg / kg before and after irradiation, respectively. Rats in the irradiation group were irradiated with 4.5 Gy of 60Co γ ray once (except for the normal control group) Do not give any treatment. Dynamic changes of body weight and peripheral blood WBC within 4 weeks after irradiation were observed dynamically. The pathological changes of bone marrow in each group were compared at 1 week and 4 weeks after irradiation. The liver of each group was measured by colorimetric method Tissue anti-oxidase activity. Results Compared with the normal control group, the body weight of the irradiated rats was significantly reduced (P <0.05). On the 1st, 4th and 5th days after irradiation, the WBC in the T1 prevention group and the T1 treatment group were significantly higher than those in the radiation group (P <0.05). There was no significant difference between the T1 prevention group and the T1 treatment group. From the 6th day, the WBC entered the recovery phase. There was no significant difference in WBC except the normal control group (P> 0.05) After 1 week, the hematopoietic cells in the radiation group decreased significantly with adipose tissue hyperplasia, and the injury in the T1 prevention group and the T1 treatment group was relatively light. In the T1 prevention group, the liver homogenate CAT, GSH-Px activity was significantly higher than that in the radiation group, MDA level was significantly lower (4.06 ± 0.19vs5.61 ± 0.46, P <0.05). Conclusion T1 can slow the declining rate of WBC after irradiation, advance the recovery time of WBC and protect the bone marrow from radiation. The mechanism may be related to the elimination of excess harmful free radicals in the body.