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以正常培养和油酸诱导培养的人肝癌细胞系(human hepatocellular liver carcinoma cell line,Hep G2)细胞为模型,通过测定普洱茶茶色素对Hep G2细胞内甘油三酯(triglyceride,TG)和总胆固醇(total cholesterol,TC)含量,细胞中脂肪酸合成酶(fatty acid synthase,FAS)、固醇调节元件结合蛋白1c(sterol regulatory element binding protein 1c,SREBP-1c)、三磷酸腺苷结合转运子A1(ATP binding cassette transporter A1,ABCA1)、胆固醇7α-羟化酶(cholesterol 7α-hydroxylase,CYP7A1)的转录水平,磷酸化腺苷酸活化蛋白激酶(phospho-AMPactivated protein kinase,p-AMPK)的蛋白表达水平研究普洱茶茶色素的减肥降脂作用机制。结果显示,普洱茶茶色素能明显降低油酸诱导的Hep G2细胞模型中TG和TC含量,作用程度依赖于普洱茶的作用质量浓度。但对正常培养的Hep G2的TG、TC作用影响不显著。经过普洱茶茶色素作用油酸诱导Hep G2细胞24 h后,能显著下调细胞的FAS和SREBP-1c的m RNA表达水平(P<0.05),显著上调ABCA1的转录水平(P<0.05),且使CYP7A1的转录水平呈上升趋势,并显著上调p-AMPK蛋白的表达量(P<0.05)。因此,普洱茶茶色素可通过调控上述调控因子和酶的表达而改善油酸诱导下Hep G2细胞的脂质代谢水平。
Hep G2 cells were cultured in normal culture and oleic acid-induced culture. The effects of Pu-erh tea pigment on triglyceride (TG) and total cholesterol total cholesterol (TC), the content of fatty acid synthase (FAS), sterol regulatory element binding protein 1c (SREBP-1c), ATP binding cassette transporter A1 A1, ABCA1), cholesterol 7α-hydroxylase (CYP7A1) and protein expression of phospho-AMP activated protein kinase (p-AMPK) The mechanism of weight loss and lipid-lowering. The results show that Pu-erh tea pigment can significantly reduce the oleic acid-induced Hep G2 cell model of TG and TC content, the degree of effect depends on the role of Pu-erh tea quality concentration. However, the effects of TG and TC on normal cultured Hep G2 were not significant. After 24 hours of oleic acid-induced oleic acid-induced Pu-erh tea pigment, the expression of m RNA in FAS and SREBP-1c cells was down-regulated significantly (P <0.05), and the ABCA1 transcription level was significantly increased The transcriptional level of CYP7A1 was up-regulated and the expression of p-AMPK protein was significantly up-regulated (P <0.05). Therefore, Pu-erh tea pigment can improve oleic acid-induced lipid metabolism in Hep G2 cells by regulating the expression of these regulatory factors and enzymes.