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目的探讨外周血树突状细胞(DC)亚群相对数量与慢性乙型肝炎(简称慢乙肝)患者血清乙型肝炎病毒(HBV)DNA水平和肝脏病理炎症损伤程度问的关系。方法定量聚合酶链反应法检测患者血清HBV DNA,利用流式细胞仪检测外周血DC亚群。结果血清HBV DNA<106拷贝/ml慢乙肝患者外周血DC2相对数量显著高于HBV DNA≥106拷贝/ml患者和健康者(P<0.05),而后两组间DC2的差异无统计学意义;上述三组中DC1相对数量差异无统计学意义;外周血DC亚群相对数与患者临床型别和肝内炎症损伤程度无关。结论外周血DC2亚群的升高与慢乙肝患者体内HBV低水平复制相关,提示DC2 可能在抑制HBV复制中发挥作用;外周血DC亚群相对数与肝组织炎症程度不相关。
Objective To investigate the relationship between the relative number of peripheral blood dendritic cells (DC) subsets and the serum hepatitis B virus (HBV) DNA level and the severity of hepatic pathological inflammation in patients with chronic hepatitis B (chronic hepatitis B). Methods Serum HBV DNA was detected by quantitative polymerase chain reaction (PCR) and peripheral blood DC subsets were detected by flow cytometry. Results The relative amount of DC2 in peripheral blood of patients with serum HBV DNA <106 copies / ml was significantly higher than that of HBV DNA ≥ 106 copies / ml (P <0.05), but there was no significant difference in DC2 between the two groups There was no significant difference in the relative numbers of DC1 among the three groups. The relative number of DC subsets in peripheral blood was not related to the clinical type and degree of intrahepatic inflammatory injury. Conclusions The elevated DC2 subpopulation in peripheral blood is associated with low level HBV replication in patients with chronic hepatitis B, suggesting that DC2 may play a role in inhibiting HBV replication. The relative numbers of DC subsets in peripheral blood are not correlated with the degree of liver inflammation.