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利用Au纳米粒子作为辣根过氧化物酶(HRP)标记抗体的载体,结合电堆积预富集技术,发展了一种基于场放大进样及Au纳米粒子双重富集的毛细管电泳电化学免疫分析技术用于大肠杆菌的检测.大肠杆菌与酶标抗体免疫反应后直接进行场放大进样预富集,免疫样品快速迁移并堆积在毛细管入口端,同时带负电荷的金纳米粒子向阳极端迁移,在样品与缓冲溶液的界面处吸附样品离子.金纳米粒子作为多酶载体使检测信号进一步放大.以标记在抗体上的HRP催化H2O2氧化邻苯二胺产生的电流信号来检测大肠杆菌.同常规电动进样毛细管电泳相比,该双重富集技术可使灵敏度提高1400倍.该方法对大肠杆菌检测的线性范围为2.0～2000.0 cfu mL-1,检出限为1.0 cfu mL-1,实现了对扇贝样品中大肠杆菌的快速、灵敏检测.
Au nanoparticles were used as carriers of horseradish peroxidase (HRP) -labeled antibody and electrocapillaries pre-enrichment technology was developed to develop a capillary electrophoresis based electrochemical immunoassay based on field-amplified injection and double enrichment of Au nanoparticles. Technology for the detection of Escherichia coli.E.coli and enzyme-labeled antibody directly after the field-amplified injection pre-enrichment, rapid migration of immune samples and deposited at the capillary inlet, while the negatively charged gold nanoparticles migrate to the anode, The adsorption of sample ions at the interface between the sample and the buffer solution.Gold nanoparticle as a multi-enzyme vector to further amplify the detection signal.A labeled antibody on the HRP catalytic oxidation of o-phenylenediamine current signal to detect E. coli.As with the conventional Compared with electrokinetic capillary electrophoresis, the dual enrichment technique can increase the sensitivity by 1400. The linear range of this method for detection of Escherichia coli is 2.0-2000.0 cfu mL-1 and the detection limit is 1.0 cfu mL-1 Fast, sensitive detection of E. coli in scallop samples.