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目的:采用RT-PCR的方法反转录链脲佐菌素(STZ)诱导的糖尿病大鼠肾皮质中氧化低密度脂蛋白内皮受体(LOX-1)和细胞间黏附分子1(ICAM-1)基因,并予半定量法测定其mRNA含量,以探讨两者在糖尿病肾病的发生和发展中可能参与的机制。方法:22只成年SD大鼠,选择其中18只注射STZ成糖尿病模型,24h后12只大鼠血糖浓度大于10mmol/L定为糖尿病模型组(DM组),再取其中4只给予注射胰岛素作为治疗对照组(TC组)。另选4只作为正常对照组(NC组),每4周测定尿白蛋白定量,14周取肾皮质匀浆后采用RT-PCR检测其中LOX-1和ICAM-1mRNA的表达,并利用半定量法测定其含量变化。结果:DM组的尿白蛋白定量自第4周后高于NC组,差异有显著性,但与TC组差异无显著性;DM组肾皮质内LOX-1和ICAM-1mRNA的含量与NC组比较显著上调,与TC组差异无显著性;DM组LOX-1和ICAM-1mRNA的含量与尿白蛋白定量存在相关性。结论:LOX-1和ICAM-1可能参与了糖尿病肾病的发生与发展。
OBJECTIVE: To reverse the expression of LOX-1 and ICAM-1 in renal cortex induced by streptozotocin (STZ) in rats by reverse transcription-polymerase chain reaction (RT-PCR) ) Gene, and semi-quantitative determination of its mRNA content in order to explore the two may be involved in the pathogenesis and development of diabetic nephropathy mechanism. Methods: Twenty-two adult Sprague-Dawley rats were selected and injected with STZ into the model of diabetes. After 12 hours, the blood glucose of 12 rats was higher than 10 mmol / L, and the diabetic group was given DM as the model group. Treatment control group (TC group). Another 4 rats were selected as the normal control group (NC group). Urinary albumin was quantified every 4 weeks. The expression of LOX-1 and ICAM-1 mRNA was detected by RT-PCR after 14 weeks of renal cortical homogenization. Semiquantitative Determination of its content changes. Results: The urinary albumin in DM group was higher than that in NC group after 4 weeks, but there was no significant difference between DM group and TC group. The contents of LOX-1 and ICAM-1 mRNA in DM group were significantly higher than those in NC group Compared with TC group, there was no significant difference between the two groups. The content of LOX-1 and ICAM-1 mRNA in DM group was correlated with urinary albumin. Conclusion: LOX-1 and ICAM-1 may be involved in the occurrence and development of diabetic nephropathy.