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运用反相高效液相(RP-HPLC)色谱法测定槐米中芦丁、槲皮素等黄酮类化合物.采用InertSustain C18色谱柱,柱温25℃,甲醇︰水=70︰30为流动相,流速0.8 mL/min等度洗脱,检测波长254nm.实验结果表明,各组分的色谱峰均达到基线分离,该方法重复性好,灵敏度高,回收率高.对超声提取和热碱提取-酸沉淀提取的样品前处理方法进行比较,采用HPLC法考察不同前处理方法对提取成分及含量的影响,结果表明,超声提取无需加热,比热碱提取-酸沉淀法提取时间短,面积归一法分析超声60%乙醇提取产物中芦丁占98.6%,未检测到槲皮素.
Determination of rutin, quercetin and other flavonoids in Flos Sophorae by reversed-phase high performance liquid chromatography (RP-HPLC) was performed on an InertSustain C18 column with a mobile phase of methanol: water = 70:30 at 25 ℃, Flow rate of 0.8 mL / min isocratic elution, detection wavelength 254nm.Experimental results show that the chromatographic peaks of each component to achieve baseline separation, the method is good repeatability, high sensitivity, high recovery.Ultrasonic extraction and hot alkali extraction - The results showed that ultrasonic extraction did not require heating, and the extraction time was shorter than that of acid-base precipitation method and the area was normalized Law analysis of 60% ethanol extract of rutin accounted for 98.6%, did not detect quercetin.