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以平邑甜茶幼苗为试材,研究了镉胁迫下幼苗叶片和根系膜脂肪酸构成、活性氧、脂氧合酶和丙二醛含量的变化.结果表明:氯化镉处理后7～12h,脂肪酸种类及其相对含量变化最为明显.处理后7h,叶片和根系脂肪酸不饱和水平升至最高,含量分别达82.82%和72.43%;叶片可检测到的脂肪酸在处理后12h由11种增至14种,根系则在处理17h后由4种增至6种.O2.-产生速率在处理3h、H2O2含量在处理7h时升至最高,丙二醛含量和脂氧合酶活性则随着处理时间的延长逐渐增加.镉胁迫通过诱导活性氧和脂氧合酶来改变平邑甜茶脂肪酸构成,并引起脂质过氧化;镉处理12h前,脂质过氧化是活性氧和脂氧合酶的共同结果;但处理12h后,脂质过氧化加剧主要在于脂氧合酶活性的持续增加.
The contents of fatty acids, reactive oxygen species, lipoxygenase and malondialdehyde in leaves and roots of cadmium stress-treated seedlings of Malus hupehensis were compared with those of the control under cadmium stress.The results showed that after 7-12 hours of cadmium chloride treatment, The content of fatty acids in leaves and roots increased to 82.82% and 72.43% respectively at 7h after treatment, and the contents of detectable fatty acids increased from 11 to 14 at 12h after treatment , And the root increased from 4 to 6 after 17h treatment.O2.- The rate of H2O2 increased to the maximum at 7h after treatment and the malondialdehyde content and lipoxygenase activity increased with the treatment time Prolonged and gradually increased.Cd stress induces reactive oxygen species and lipoxygenase to change the composition of Fatty Acid and induce lipid peroxidation.Phyrogen peroxide is a common result of reactive oxygen species and lipoxygenase 12 h before cadmium treatment However, the increase of lipid peroxidation was mainly due to the continuous increase of lipoxygenase activity after 12 hours of treatment.