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目的:评估乳牙及年轻恒牙牙髓内的血管内皮生长因子受体-2(VEGFR-2)表达以及VEGFR-2阳性细胞的空间分布。方法:10颗健康的乳牙和10颗健康年轻恒牙采用抗VEGFR-2抗体进行免疫组化的方法并借助Image-Pro-Plux软件分析系统测量牙髓细胞中VEGFR-2的光密度值,进行统计学分析。结果:乳牙及年轻恒牙的牙髓血管内皮细胞对VEGFR-2的免疫染色均为阳性。在乳牙牙髓中VEGFR-2阳性细胞趋向于靠近成牙本质细胞层,年轻恒牙中VEGFR-2的免疫染色的空间分布更加一致。乳牙的牙髓血管内皮细胞胞浆中VEGFR-2免疫组化染色阳性,且表达高于年轻恒牙,差异有统计学意义(P<0.05),结论:VEGFR-2在乳牙及年轻恒牙牙髓的血管内皮细胞均能表达,表明这些细胞能够对由血管内皮生长因子调节的形态变化及成活信号产生反应。
Objective: To evaluate the expression of vascular endothelial growth factor receptor-2 (VEGFR-2) and the spatial distribution of VEGFR-2 positive cells in dental pulp of primary teeth and young permanent teeth. Methods: Immunohistochemistry was performed on 10 healthy deciduous teeth and 10 healthy young permanent teeth using anti-VEGFR-2 antibody and the optical density of VEGFR-2 in dental pulp cells was measured by Image-Pro-Plux software analysis system Statistical analysis. Results: Immunofluorescence of VEGFR-2 was positive in dental pulp vascular endothelial cells of primary teeth and young permanent teeth. VEGFR-2 positive cells tended to be closer to the odontoblast layer in deciduous dental pulp and the spatial distribution of VEGFR-2 immunostaining was more consistent in young permanent teeth. The positive rate of VEGFR-2 in the cytoplasm of dental pulp vascular endothelial cells of primary teeth was higher than that of young permanent teeth (P <0.05), and the difference was statistically significant (P <0.05). Conclusion: VEGFR- Of the vascular endothelial cells were able to express, indicating that these cells can be regulated by the vascular endothelial growth factor morphological changes and survival signal response.