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目的本实验用去卵巢大鼠建立更年期动物模型,给予不同剂量外源性雌二醇(E2),探讨E2对去卵巢大鼠心肌细胞和血管内皮细胞保护作用及机制。方法用RIA测定血清E2浓度和内皮素(ET)浓度;用酶法检测血清中NO浓度。用免疫组织化法检测心血管细胞雌激素受体的ER mRNA表达的观察。结果血清中NO与E2补充量呈正相关,ET与E2补充量呈负相关。ER mRNA在OVX组大鼠心肌细胞的胞浆中呈弱阳性表达,补充大剂量组大鼠心肌细胞的胞浆呈阳性表达。结论去卵巢后补充E2可使心血管中ER表达增加,提示绝经后心血管中ER含量减少可能是导致绝经后女性心血管病发病率迅速上升的重要机制之一。去卵巢后补充E2可使血清中NO含量增加,ET含量减少,说明血中E2可以影响或调节心血管内皮细胞分泌NO、和ET的含量。
Objective To establish a model of climacteric animal in ovariectomized rats and exogenous estradiol (E2) at different doses to explore the protective effect and mechanism of E2 on cardiomyocytes and vascular endothelial cells in ovariectomized rats. Methods Serum E2 concentration and ET concentration were determined by RIA. Serum NO concentration was detected by enzymatic method. Detection of ER mRNA expression in estrogen receptor of cardiovascular cells by immunohistochemistry. Results There was a positive correlation between serum NO and E2 supplementation and a negative correlation between ET and E2 supplementation. ER mRNA was weakly positive in the cytoplasm of cardiomyocytes in OVX group, and positive in cytoplasm of cardiomyocytes in high dose group. Conclusions Overexpression of E2 after ovariectomy can increase the expression of ER in cardiovascular system, suggesting that the decrease of ER in cardiovascular system after menopause may be one of the important mechanisms leading to the rapid increase of cardiovascular disease in postmenopausal women. After ovariectomized with E2 can increase the serum NO content, ET content decreased, indicating that E2 in the blood can affect or regulate the secretion of endothelial cells of endothelial NO, and ET content.