论文部分内容阅读
从慢性感染者血浆和肝脏提纯的乙型肝炎病毒(HBV)的特异性核酸能掺入~(32)P 标记的脱氧核苷酸。硫酸铯密度梯度平衡离心表明,在1.45~1.60g/cm~3浮力密度处存在HBV DNA-RNA 杂交分子。血浆 HBV 杂交分子的密度是1.57g/cm~3,而肝内 HBV 杂交分子分别是1.57、1.52g/cm~3,成带都很清楚。经 HBV 相关病毒感染的鸭子、地松鼠肝内病毒也发现 DNA—RNA 分子,密度
Specific nucleic acids of Hepatitis B virus (HBV) purified from the plasma and liver of chronically infected individuals can incorporate ~ (32) P-labeled deoxynucleotides. Cesium sulfate density gradient centrifugation showed that HBV DNA-RNA hybrids existed at buoyant densities of 1.45 to 1.60 g / cm ~ 3. Plasma HBV hybrids have a density of 1.57 g / cm ~ 3, while intrahepatic HBV hybrids are 1.57 and 1.52 g / cm ~ 3, respectively. Ducks infected with HBV-related virus, ground squirrel liver virus also found DNA-RNA molecules, density