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代谢组学是近年来快速发展起来的一门学科,具有高灵敏度的色谱质谱联用技术是其主要的分析手段之一。代谢组学的主要研究对象为尿液、血浆及胆汁等,其中尿液由于受到饮水量等不可控因素的影响,导致内源性代谢产物的浓度发生改变,因此必须进行归一化处理以消除这种差异。本文综述了4种基于色谱质谱联用的归一化方法:肌酐归一化、峰面积归一化、渗透浓度归一化及尿液体积归一化,并分别阐述了其优缺点,希望能对相关的研究工作提供参考。
Metabonomics is a rapidly developing discipline in recent years. Highly sensitive chromatographic-mass spectrometry (MS / MS) technology is one of the major analytical tools. Metabolomics of the main research object is urine, plasma and bile, etc., of which urine due to water and other uncontrollable factors, resulting in endogenous metabolites concentration changes, it must be normalized to eliminate This difference. In this paper, four kinds of normalization methods based on chromatographic and mass spectrometry are reviewed: normalization of creatinine, normalization of peak area, normalization of osmotic concentration and normalization of urine volume, and their advantages and disadvantages respectively, Provide relevant reference for the research work.