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使用柠檬酸三钠还原法制备胶体金颗粒,在pH值7.6,蛋白用量10～15μg/mL条件下,制备形成4种葫芦科植物病毒多克隆抗体的稳定胶体金蛋白复合物;设计了双向复合试纸条,使用微定量喷头在试纸条两侧的硝酸纤维素膜上分别喷2条病毒检测线和1条质控线,组装制成免疫层析检测试纸条。结果表明经过条件优化后制备的双向复合试纸条特异性好,可在10min内同时检测4种葫芦科植物病毒,且对不同病毒阳性材料的检测灵敏度可达到稀释103倍以上。
Colloidal gold particles were prepared by the trisodium citrate reduction method, and the stable colloidal gold protein complexes forming 4 polyclonal antibodies to Cucurbitaceae virus virus were prepared under the conditions of pH 7.6 and 10 ~ 15μg / mL of protein. Test strip, the use of micro-quantitative nozzle in the test strips on both sides of the nitrocellulose membrane were sprayed two virus detection lines and a control line, the assembly made of immunochromatographic test strip. The results showed that the bi-directional composite strips prepared by the optimized conditions were of good specificity and could detect 4 Cucurbitaceae viruses simultaneously within 10 min, and the detection sensitivity of different virus positive materials could be more than 103 times dilution.