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目的:观察复方女贞益母黄芪汤(CNT)对小鼠免疫功能的影响。方法:选用健康NIH小鼠,按体重随机分成5组:空白对照组、阳性对照组(给予强的松20 mg/kg.d)、复方女贞益母黄芪汤高(8.92 g生药/kg.d)、中(4.46 g生药/kg.d)、低(2.23g生药/kg.d)剂量组。分别灌胃给药,空白对照组灌胃等体积蒸馏水,1次/d,连续7 d。分别测定免疫蛋白IgG、IgM、IgA、补体C3、循环免疫复合物(C IC)的含量;计算脾脏指数和胸腺指数(mg/g);观察复方女贞益母黄芪汤对小鼠腹腔巨噬细胞吞噬功能的影响,并以鸡红细胞(CRBC)诱发小鼠迟发型变态反应(DTH),观察其对小鼠足垫肿胀的作用。结果:复方女贞益母黄芪汤能显著降低小鼠血清中IgG、IgM、IgA、C IC的含量,升高补体C3的含量,明显抑制小鼠的DTH和腹腔巨噬细胞的吞噬功能,使吞噬百分率和吞噬指数减少(P<0.05或P<0.01);各剂量组对小鼠的胸腺和脾脏指数均无明显影响(P>0.05)。结论:复方女贞益母黄芪汤具有免疫抑制的药理作用。
OBJECTIVE: To observe the effect of Compound Ligustrum eutrophic yin decoction (CNT) on immune function in mice. METHODS: Healthy NIH mice were randomly divided into five groups according to body weight: blank control group, positive control group (prednisone 20 mg/kg.d) and compound Ligustrum Yimukang Decoction (8.92 g crude drug/kg. d) Medium (4.46 g crude drug/kg.d), low (2.23 g crude drug/kg.d) dose groups. The rats were intragastrically administered and the control group was given equal volume of distilled water once a day for 7 days. The contents of immune proteins IgG, IgM, IgA, complement C3, and circulating immune complexes (CIC) were measured; the spleen index and thymus index (mg/g) were calculated; and the macrophage in the peritoneal cavity of the mice was observed with Ligustrum lucidum compound Yimu Huangqi Decoction. Effects of cell phagocytosis and induced late-type hypersensitivity (DTH) in mice with chicken erythrocytes (CRBC) were used to observe the effect of paw swelling in mice. Results: Compound Ligustrum Yimu Huangqi Decoction can significantly reduce the serum IgG, IgM, IgA, C IC content, increase the content of complement C3, and significantly inhibit the phagocytosis of DTH and peritoneal macrophages in mice. The percentage of phagocytosis and phagocytic index decreased (P<0.05 or P<0.01); there was no significant effect on thymus and spleen index of mice in each dose group (P>0.05). Conclusion: Compound Ligustrum Yimu Huangqi Decoction has the pharmacological effects of immunosuppression.