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用两种特异的限制性核酸内切酶同时酶切含目的基因的DNA片段与质粒,可以有效的降低或防止目的基因和质粒表达载体在酶切后的末端发生任意连接;双抗生素间接平板筛选,得到含有目的基因的质粒。
Using two specific restriction endonucleases to simultaneously digest the DNA fragments and plasmids containing the target gene can effectively reduce or prevent the target gene and the plasmid expression vector from being arbitrarily connected at the end of the digestion; indirect antibiotic screening To obtain a plasmid containing the gene of interest.