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采用pH漂移技术(pH drifttechnique) ,对海带雌配子体克隆的碳素营养进行了研究。结果表明 ,海带雌配子体对无机碳的利用能力较弱 ,其pH补偿点为8.85 ,CO2 补偿点为1.635μmol/L。与HCO- 3 利用有关的抑制剂 :Acetazolamide(Az) ,4′4′ diisothiocyanatosilbene 2 ,2 disulfonicacid(DIDS) ,4 acetamido 4′ isothiocyano 2 ,2′ stibene disulfonate(SITS)均未对海带雌配子体无机碳的利用产生任何影响 ,进一步说明了海带雌配子体只以游离的CO2 为无机碳源 ,不能利用HCO- 3;同时还发现 ,海带雌配子体利用无机碳的过程中 ,并未引起碱度的变化 ,从而可以用 pH指示密闭培养过程中无机碳的变化。
The carbon nutrition of the female gametophyte clone was studied using pH drifttechnique. The results showed that the utilization ability of female gametophyte for inorganic carbon was weak with the pH compensation point of 8.85 and the CO2 compensation point of 1.635 μmol / L. No inhibitor of the utilization of HCO-3: Acetazolamide (Az), 4’4 ’diisothiocyanatosilbene 2, 2 disulfonicacid (DIDS), 4 acetamido 4’ isothiocyano 2, 2 ’stibene disulfonate (SITS) The results showed that the female gametophyte of kelp used only free CO2 as inorganic carbon source and could not utilize HCO-3. At the same time, it was found that the change of alkalinity did not result in the utilization of inorganic carbon in the female gametophyte of kelp, PH can be used to indicate the change of inorganic carbon in the closed culture.