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目的:建立人尿中奥美拉唑代谢物的HPLC测定方法,同时测定了单剂量口服40 mg奥美拉唑后不同时间奥美拉唑代谢物的尿中浓度。方法:采用DiamonsiL ODS C_(18)柱(150 mm×4.6 mm,5μm);流动相:甲醇-冰醋酸缓冲液(68:32,缓冲液中含1%三乙胺);流速:1.0 ml·min~(-1),检测波长:302 nm。结果:奥美拉唑代谢物(M-1)回归方程为Y=4.44×10~4C+ 292.51,r=0.9998,线性范围为0.142～28.4μg·ml~(-1);代谢物(M-2)回归方程为Y=1.52×10~5C+75.22,r=0.999 5,线性范围为0.18～36.0μg·ml~(-1)。方法回收率为99.41%～102.26%。结论:本法灵敏度高,重现性好,专属性强,适合奥美拉唑代谢产物的药动学研究。
OBJECTIVE: To establish a HPLC method for the determination of omeprazole metabolites in human urine and to determine urinary concentrations of omeprazole metabolites at different times after a single oral dose of 40 mg omeprazole. METHODS: DiamonsiL ODS C 18 column (150 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of methanol-glacial acetic acid buffer (68:32, 1% triethylamine in buffer) min ~ (-1), detection wavelength: 302 nm. Results: The regression equation of omeprazole metabolite (M-1) was Y = 4.44 × 10-4C + 292.51, r = 0.9998 with a linear range of 0.142-28.4μg · ml -1. The metabolites (M-2 ) The regression equation was Y = 1.52 × 10 ~ 5C + 75.22, r = 0.999 5, the linear range was 0.18 ~ 36.0μg · ml -1. The recovery rate was 99.41% ~ 102.26%. Conclusion: This method has high sensitivity, good reproducibility and specificity and is suitable for pharmacokinetic study of omeprazole metabolites.