论文部分内容阅读
本文提出了用乙腈沉淀血液中蛋白、RP柱吸附有机杂质、Ag柱去除氯离子后,氢氧化钾溶液梯度淋洗,经AS17-c保护柱及AS17-c分离柱分离,抑制电导检测器检测血液中亚硝酸根离子。亚硝酸根离子在1.5u mol·L~(-1)~300u mol·L~(-1)范围内与对应峰面积呈线性关系,方法检测限(3s/k)小于3.0u mol·L~(-1),加标回收率在87.1%-97.8%之间,RSD为2.7%。
In this paper, the protein in blood was precipitated by acetonitrile, the organic impurities were adsorbed on RP column, the chloride ion was removed by Ag column, the gradient elution was carried out by potassium hydroxide solution, and separated by AS17-c guard column and AS17-c column to inhibit the detection of conductivity detector Blood nitrite ion. The results showed that the detection limits (3s / k) were less than 3.0u mol·L ~ (-1) in the range of 1.5u mol·L ~ (-1) ~ 300u mol·L ~ (-1) (-1). The spiked recoveries ranged from 87.1% to 97.8% with a RSD of 2.7%.