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采用微胚早期转移技术，将接种后２周的花药连同微胚一起转移到分化培养基，可提高分化成苗率１０～１２倍，且减轻了胚状体转移的麻烦。利用５０ｋｇ／Ｌ的秋水仙碱溶液处理１～２ｍｍ的胚状体６～７２小时，可在一定范围内提高可育株率，其中处理２４小时效果最好，在不降低分化成苗率的前提下，可育株率由对照的３２．３％提高到７１．４％。将微胚早期转移与染色体早期加倍相结合，在微胚转移前一天向原培养基中滴加１ｍｌ５０ｍｇ／Ｌ的秋水仙碱溶液，在保持同样出胚率和分化率的基础上，可育株率由３９．４％提高到５８．３％，自交结实株率由１７．９％提高到３７．４％。
Using the micro-embryo transfer technology, the anther transferred to the differentiation medium together with the micro-embryos two weeks after the inoculation can increase the rate of differentiation into the seedlings by 10 to 12 times and relieve the trouble of embryoid body transfer. Using 50kg / L colchicine solution for 1 ~ 2mm embryoid body for 6 ~ 72 hours, can be increased within a certain range of fertile plant rate, which deal with the best 24 hours, without reducing the rate of differentiation into seedling premise , The fertile plant rate increased from 32.3% of the control to 71.4%. The early embryo transfer of micro-embryos combined with early chromosome doubling, the day before the micro-embryo transfer to the original medium 1ml50mg / L colchicine solution, while maintaining the same embryogenic rate and differentiation rate, the fertile plant rate Increased from 39.4% to 58.3%, and the rate of selfed selfing increased from 17.9% to 37.4%.