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探讨直接利用心肌匀浆测定肌浆网Ｃａ２＋－ＡＴＰ酶的方法，并观察左室肥厚时肌浆网Ｃａ２＋－ＡＴＰ酶的变化。采用ｐ－硝苯基磷酸（ｐ－ＮＰＰ）法建立ＳＤ大鼠心肌匀浆肌浆网Ｃａ２＋－ＡＴＰ酶检测的最佳条件；取６周龄自发性高血压大鼠（ＳＨＲ）及对照组饲养２４周，观察血压、左室肥厚及心肌肌浆网Ｃａ２＋－ＡＴＰ酶活力。结果在心肌匀浆中建立了肌浆网Ｃａ２＋－ＡＴＰ酶活力测定的最佳条件；并发现ＳＨＲ左室肥厚时肌浆网Ｃａ２＋－ＡＴＰ酶活力较对照组明显下降（Ｐ＜０．０１）。
To explore the direct use of myocardial homogenate sarcoplasmic reticulum Ca2 + -ATP enzyme method and observed sarcoplasmic reticulum Ca2 + -ATP enzyme changes in left ventricular hypertrophy. The optimal conditions of sarcoplasmic reticulum Ca2 + -ATPase in SD rat myocardial tissue were established by p-nitrophenyl phosphate (p-NPP) method. Six-week-old spontaneously hypertensive rats (SHR) and control group were bred After 24 weeks, blood pressure, left ventricular hypertrophy and cardiac sarcoplasmic reticulum Ca2 + -ATPase activity were observed. Results The optimum condition of sarcoplasmic reticulum Ca2 + -ATPase activity was established in myocardial homogenate. It was found that Ca2 + -ATPase activity in sarcoplasmic reticulum was significantly decreased in SHR group compared with control group (P <0.01).