目的:探究ghrelin在脑缺血再灌注损伤后神经修复过程中对神经元、星形胶质细胞及其功能网络联系的作用及机制。方法:培养离体脑片;采用氧糖剥夺/复氧复糖(OGD/R)模型模拟脑缺血再灌注损伤;OGD/R损伤后给予ghrelin,连续观察7 d其对神经修复过程的作用;免疫组化染色观察细胞数量、形态及生长状态变化;Western blot检测蛋白表达;PCR检测mRNA表 Objective: To investigate the effect and mechanism of ghrelin on neurons, astrocytes and their functional networks during neural repair after cerebral ischemia-reperfusion injury. Methods: The isolated rat brain slices were cultured. OGD / R model was used to simulate cerebral ischemia-reperfusion injury. The ghrelin was given after OGD / R injury and the effect was observed continuously for 7 days Immunohistochemistry was used to observe the changes of cell number, morphology and growth status; Western blot was used to detect the protein expression;