应用淋巴细胞杂交瘤技术获得１０株抗重组人肿瘤坏死因子－α（ｒＨｕＴＮＦ－α）ＭｃＡｂ。７株ＭｃＡｂ为ＩｇＧ１，２株为ＩｇＧ２ａ，１株为ＩｇＧ２ａ。１０株ＭｃＡｂ与淋巴毒素（ＬＴ或ｒＨｕＴＮＦ－β）、ｒＨｕＩＬ－６和载体菌菌体蛋白均无交叉反应。其中７株ＭｃＡｂ具有中和活性，７株ＭｃＡｂ能识到天然的ＴＮＦ－α。Ｇ１１和Ｅ６ＭｃＡｂ应用ＡＢＣ法免疫组化染色肝病患者组织细胞片得到明确的阳性结果。用２株识别不同表位的ＭｃＡｂ建立了一步夹心ＥＬＩＳＡ法，检测ＴＮＦ－α的敏感性可达到１００ｐｇ／ｍｌ左右。 Ten anti-recombinant human tumor necrosis factor-α (rHuTNF-α) McAbs were obtained using lymphocyte hybridoma technique. Seven McAbs were IgG1, two were IgG2a and one was IgG2a. Ten McAbs did not cross-react with lymphotoxin (LT or rHuTNF-β), rHuIL-6 and the carrier bacterial cell proteins. Among them, 7 McAbs had neutralizing activity, and 7 McAbs could recognize native TNF-α. G11 and E6McAb ABC method immunohistochemical staining of liver cells in patients with clear positive results. Two McAbs with different epitopes were used to establish a one-step sandwich ELISA. The sensitivity of detecting TNF-α was about 100pg / ml.