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目的探讨胃癌组织中抗凋亡蛋白livin的表达及生物学意义。方法采用原位杂交技术和免疫组织化学方法检测49例胃癌组织和20例正常胃组织中的livin基因及蛋白表达。结果 49例胃癌组织中,livin蛋白表达阳性率为49.0%(24/49),livinmRNA表达阳性率为46.9%(23/49),正常胃组织中呈低表达,阳性率为20%(4/20)。Livin表达与肿瘤大小及淋巴结转移有关(P﹤0.05)。结论 Livin基因及蛋白在胃癌中表达上调,提示在胃癌的发生、发展中起重要作用,有望成为一种有效敏感的肿瘤标记物,可作为判断胃癌预后的新分子标记物和胃癌诱导凋亡治疗的新靶点。
Objective To investigate the expression and biological significance of anti-apoptotic protein livin in gastric cancer. Methods The expression of livin gene and protein in 49 cases of gastric carcinoma and 20 cases of normal gastric tissue were detected by in situ hybridization and immunohistochemistry. Results The positive rates of livin protein in 49 cases of gastric carcinoma were 49.0% (24/49), 46.1% (23/49) in livin mRNA and 20% (4 / 20). Livin expression was correlated with tumor size and lymph node metastasis (P <0.05). Conclusion The upregulation of Livin gene and protein in gastric cancer suggests that Livin gene and protein play an important role in the occurrence and development of gastric cancer and may be an effective and sensitive tumor marker. It may be a new molecular marker for gastric cancer prognosis and induction of apoptosis in gastric cancer New target.