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目的:研究蔓荆子总黄酮(FVTF)抑制人肝细胞癌自我更新作用及其机制是否涉及AMPK/Akt信号传导。方法:肿瘤球形成法测定FVTF对肝细胞癌SMMC-7721和MHCC97H细胞系球形成率的影响。Western blot分析FVTF处理SMMC-7721细胞AMPK和Akt磷酸化水平。结果:FVTF显著降低SMMC-7721和MHCC97H细胞系球形成率,呈浓度依赖性。不同浓度FVTF(1.0、2.0、4.0μg/m L)处理SMMC-7721细胞24 h,AMPK磷酸化水平增高;Akt磷酸化水平下降。结论:FVTF抑制肝细胞癌自我更新作用与其激活AMPK抑制Akt活性相关。
Objective: To investigate whether VTF inhibits the self-renewal of human hepatocellular carcinoma and whether its mechanism involves AMPK / Akt signaling. METHODS: The effect of FVTF on the rate of spheroid formation in hepatocellular carcinoma SMMC-7721 and MHCC97H cell lines was determined by tumor spheres formation. Western blot analysis of FVTF SMMC-7721 cells treated with AMPK and Akt phosphorylation level. Results: FVTF significantly reduced the rate of spherical SMMC-7721 and MHCC97H cell formation in a concentration-dependent manner. The SMMC-7721 cells treated with different concentrations of FVTF (1.0,2.0,4.0μg / ml) for 24 h increased the phosphorylation of AMPK and the phosphorylation of Akt. Conclusion: FVTF can inhibit the self-renewal of hepatocellular carcinoma and its effect on AMPK activation and Akt activation.