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将30只SPF级雄性昆明小鼠随机分为对照组、CdSeⅠ染毒组(粒径50～100μm)、CdSeⅡ染毒组(粒径40～50 nm),每组10只。腹腔注射染毒,两染毒组染毒剂量为40 mg/kg,对照组给予等体积的生理盐水,隔天染毒30 d处死,取睾丸和附睾组织。观察小鼠精子存活率、畸形率及睾丸组织病理学变化。结果显示,两染毒组精子存活率较对照组均下降,畸形率上升(P<0.05),且CdSeⅡ组的精子存活率最低,畸形率最高。病理学观察显示染毒组小鼠睾丸出现不同程度损伤,纳米CdSeⅡ组小鼠睾丸损伤最为严重。提示纳米硒化镉对雄性小鼠的睾丸组织有严重的损伤作用,且粒径是决定纳米硒化镉毒性的主要因素,粒径越小的纳米硒化镉材料的毒性越大。
Thirty SPF male Kunming mice were randomly divided into control group, CdSe Ⅰ group (50-100 μm in diameter) and CdSe Ⅱ group (40-50 nm in diameter), with 10 mice in each group. The rats were injected intraperitoneally with a dose of 40 mg / kg. The control group was given equal volume of normal saline. The rats were sacrificed on the next day for 30 days, and the testis and epididymis were removed. Mouse sperm survival rate, deformity rate and testicular histopathological changes were observed. The results showed that the survival rate of sperm in both groups were decreased compared with the control group, the rate of deformity increased (P <0.05), and the sperm survival rate of CdSeⅡ group was the lowest, the highest deformity rate. Pathological observation showed that the testis in the exposed group showed different degrees of damage, and the mice in the CdSeⅡ group had the most severe testicular injuries. It is suggested that nano-CdSe has a serious damage on testicular tissue in male mice, and the particle size is the main factor that determines the toxicity of nano-CdSe. The smaller the particle size, the more toxic the CdSe material.