The singularly perturbed bifurcation subsystem is described,and the test conditions of subsystem persistence are deduced.By use of fast and slow reduced subsystem model,the result does not require performing nonlinear transformation.Moreover,it is shown and proved that the persistence of the periodic orbits for Hopf bifurcation in the reduced model through center manifold.Van der Pol oscillator circuit is given to illustrate the persistence of bifurcation subsystems with the full dynamic system.