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目的探讨胰升血糖素样肽-1(GLP-1)对内质网应激状态下脂肪细胞内脏脂肪素表达的调节作用。方法 3T3-L1分化成熟的脂肪细胞,经thapsigargin(Tg)诱导建立内质网应激模型。脂肪细胞经Tg和/或GLP-1孵育后,收集细胞用于总RNA的提取,收集细胞液用于ELISA分析。定量PCR检测内脏脂肪素转录水平,ELISA检测其分泌水平。内质网应激发生通过测定剪切型XBP-1(sXBP-1)表达水平增加来判断。使用细胞核因子κB(NF-κB)特异阻断剂PDTC预处理30min,观察NF-κB在GLP-1调节内脏脂肪素表达中的作用。结果 Tg诱导后sXBP-1表达增加,内脏脂肪素表达下降并呈剂量依赖性;同时给予GLP-1处理后,内脏脂肪素表达增加;PDTC降低内脏脂肪素基础水平,并未阻断GLP-1作用。结论 GLP-1可提升内质网应激状态下脂肪细胞内脏脂肪素的表达。
Objective To investigate the regulatory effect of glucagon-like peptide-1 (GLP-1) on visfatin expression in adipocytes under endoplasmic reticulum stress. Methods 3T3-L1 differentiated mature adipocytes, the thapsigargin (Tg) induced the establishment of endoplasmic reticulum stress model. After adipocytes were incubated with Tg and / or GLP-1, cells were harvested for total RNA extraction and cell extracts were collected for ELISA analysis. Quantitative PCR detection of visfatin mRNA transcription levels, ELISA test its secretion level. Endoplasmic reticulum stress was determined by measuring the increased expression of the cut XBP-1 (sXBP-1). Pretreatment with PDTC, a specific inhibitor of nuclear factor κB (NF-κB) for 30 min, was used to observe the role of NF-κB in the regulation of visfatin expression by GLP-1. Results After Tg induction, the expression of sXBP-1 was increased and the expression of visfatin was decreased and in a dose-dependent manner. At the same time, the expression of visfatin was increased after treatment with GLP-1. PDTC reduced the level of visfatin, but did not block the expression of GLP-1 effect. Conclusion GLP-1 can enhance the expression of visfatin in adipocytes under endoplasmic reticulum stress.