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稻瘟病菌基因组测序的完成有助于全新效应蛋白的挖掘和功能鉴定,而未知功能的蛋白生物信息学预测为这些蛋白的生物学功能鉴定提供了重要的理论参考.本研究利用生物信息学分析对蛋白理化性质分析及其二级结构等进行预测,并构建这四个基因与绿色荧光蛋白(GFP)融合的过表达载体.从稻瘟病数据库中下载MoS4、MoS74、MoS997、MoS1460基因序列,通过分析得到MoS4、MoS74、MoS997和MoS1460基因的ORF分别为281 bp、251 bp、267 bp和240 bp;预测MoS997蛋白属于稳定性较好的亲水蛋白,MoS4、MoS74属于不稳定的疏水蛋白,且MoS74还是一个跨膜蛋白,而MoS1460蛋白则属于不稳定的亲水性蛋白,并且获得了四个基因与绿色荧光蛋白(GFP)融合的过表达载体,并经过测序验证.研究结果为进一步探究稻瘟病菌效应蛋白基因的功能及亚细胞定位等提供科学依据.“,”The completion of the genome sequencing of Magnaporthe oryzae contributes to the excavation and functional identification of novel effect proteins, and the bioinformatics prediction of unknown functional protein provides an important theoretical reference for the identification of biological functions of these proteins.The bioinformatics analysis was used to predict the physicochemical properties of proteins and their secondary structures.The overexpression vectors of these four genes and green fluorescent protein (GFP) were constructed.The MoS4, MoS74, MoS997 and MoS1460 genes sequence were downloaded from the rice blast disease database, and the ORFs of MoS4, MoS74, MoS997 and MoS1460 genes were 281 bp, 251 bp, 267 bp and 240 bp, respectively.It was predicted that MoS997 protein was a stable hydrophilic protein, while MoS4 and MoS74 belonged to the unstable hydrophobic proteins, and MoS74 was a transmembrane protein, while the MoS1460 protein belonged to the unstable hydrophilic protein.The overexpression vector of the four genes and green fluorescent protein (GFP) was obtained, which was verified by sequencing.The results of this study could provide scientific reference for the further study on the function and subcellular localization of effect protein gene in Magnaporthe oryzae.