建立吴茱萸碱-β-环糊精的包合率测定方法。采用紫外分光光度法测定吴茱萸碱-β-环糊精的包合物中吴茱萸碱的含量,由此计算包合率。结果表明吴茱萸碱在225nm处有最大吸收,浓度在1.03—4.12μg.mL-1时与吸光度呈良好的线性关系,回归方程为:A=0.1661C+0.0145,r=0.9997(n=7);平均回收率为99.06%,RSD为0.61%。本法操作简便易行,具有良好的重现性和精密度。 The determination of evodiamine-β-cyclodextrin inclusion rate was established. The content of evodiamine in the inclusion compound of evodiamine-β-cyclodextrin was determined by UV spectrophotometry, and the inclusion ratio was calculated. The results showed that evodiamine had the maximum absorption at 225nm, and the linearity was good with the absorbance at 1.03-4.12μg.mL-1. The regression equation was: A = 0.1661C + 0.0145, r = 0.9997 (n = 7); The average recovery was 99.06% with a RSD of 0.61%. This method is easy to operate, with good reproducibility and precision.