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72只AA肉鸡适应性饲养3 d后随机分为4组,分别为空白对照组(不含黄曲霉毒素B_1(AFB_1)的空白饲料)、AFB_1低剂量组(饲料中含1 mg/kg AFB_1)、AFB_1中剂量组(饲料中含3 mg/kgAFB_1)、AFB_1高剂量组(饲料中含5 mg/kg AFB_1)。结果表明,连续饲喂高剂量AFB_128 d时,AA肉鸡肝体指数显著高于对照组(P<0.05);血清酶学指标谷丙转氨酶(ALT)、谷草转氨酶(AST)活性极显著高于对照组(P<0.01),碱性磷酸酶(ALP)活性显著高于对照组(P<0.05);肝脏总超氧化物岐化酶(T-SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性极显著低于对照组(P<0.01),丙二醛(MDA)含量极显著高于对照组(P<0.01)。病理切片结果显示,在28 d AFB_1高剂量组肉鸡肝脏发生严重病变。平均日增重结果表明,在14~21 d,AFB_1中、高剂量组AA肉鸡均极显著低于对照组(P<0.01);21~28 d,高剂量组平均日增重极显著低于对照组(P<0.01),中剂量组平均日增重显著低于对照组(P<0.05)。综上所述,连续饲喂含有5 mg/kg AFB_1的饲料28 d可以成功建立肉鸡亚慢性肝损伤模型,饲料中污染AFB_1能严重影响AA肉鸡增重。
Seventy AA broiler chickens were randomly divided into 4 groups after 3 days of adaptive feeding, which were blank control group (blank feed without aflatoxin B_1 (AFB_1)), AFB_1 low dose group (feed with 1 mg / kg AFB_1) , AFB_1 middle dose group (feed containing 3 mg / kg AFB_1) and AFB_1 high dose group (feed containing 5 mg / kg AFB_1). The results showed that the liver index of AA broilers was significantly higher than that of the control group (P <0.05) after continuous feeding of high dose of AFB - 128 d, and the activity of serum ALT and AST was significantly higher than that of the control (P <0.01). ALP activity was significantly higher than that of the control group (P <0.05). Total hepatic superoxide dismutase (T-SOD) and glutathione peroxidase -Px) significantly lower than the control group (P <0.01), and the content of malondialdehyde (MDA) was significantly higher than that of the control group (P <0.01). The results of pathology showed that severe lesions of liver were observed in the high-dose AFB_1 group at 28 d. The results of average daily gain showed that AA broilers in medium and high dose AFB_1 group were significantly lower than those in control group (P <0.01) at 14 ~ 21 d, and the average daily gain of high dose group was significantly lower than that of control group (P <0.01). The average daily gain of medium dose group was significantly lower than that of control group (P <0.05). Taken together, continuous feeding of feed containing 5 mg / kg AFB_1 for 28 days successfully established a model of subchronic liver injury in broiler chickens. Contamination of AFB_1 in feed significantly affected weight gain of AA broiler chickens.