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对已经完成全序列测定的 12个SARS病毒基因组进行了多序列比对 ,发现序列主体部分 2 970 8b具有99 82 %的相同碱基 ,除 2个序列各有 5个和 6个碱基的缺失外 ,其余部分共有 4 2个位点核苷酸碱基的差异 ,其中2 8个位点的碱基差异可引起氨基酸残基改变。利用蛋白质二级结构和跨膜螺旋预测以及蛋白质定位等生物信息学工具 ,分析了这些产生氨基酸改变部位的蛋白质构像 ,推测了可能产生的结构和功能改变 ,为进一步生物学实验提供参考。所有分析结果同时在北京大学生物信息中心抗SARS网站 (antisars.cbi.pku .edu .cn)上发布。
Multiple sequence alignment of the 12 SARS genomes that have been performed on the complete sequence was found, with 2982b of 9982% of the identical bases in the subject’s body portion, 5 and 6 bases in each of the 2 sequences In addition, the remaining part of the total of 42 nucleotide nucleotide differences, of which 28 base differences can cause changes in amino acid residues. Using bioinformatics tools such as protein secondary structure and transmembrane helix prediction and protein localization, we analyzed the protein conformations of the amino acid change sites and speculated the possible structural and functional changes, which may provide reference for further biological experiments. All analysis results are also posted on the anti-SARS website at Peking University’s Bioinformatics Center (antisars.cbi.pku .edu .cn).