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目的:探讨胃癌前病变组织中人端粒酶逆转录酶(hTERT)和p53蛋白的表达状况及其与幽门螺杆菌(Hp)根除前后的关系。方法:采用实时定量荧光RT-PCR方法检测胃粘膜中hTERT,将hTERT与GAPDH(甘油醛-3-磷酸脱氢酶)拷贝数之比的100倍作为标准化hTERT(NhTERT),用快速尿素酶法和Warthin-Starry银染色法检测Hp,同时用免疫组化检测p53蛋白的表达。结果:Hp阳性组hTERTmRNA和p53蛋白的表达率均显著高于Hp阴性亚组的表达率(均P<0.05)。Hp根除组hTERTmRNA的表达率显著低于未根除组(P<0.05),未根除组与阴性对照组之间无显著性差异。结论:在胃癌前病变阶段,Hp感染与端粒酶表达水平及p53蛋白之间有直接关系,提示端粒酶、Hp感染、p53蛋白在胃癌的发生、发展中起重要作用及Hp根除的必要性。
Objective: To investigate the expression of human telomerase reverse transcriptase (hTERT) and p53 protein in gastric precancerous lesions and their relationship with Helicobacter pylori (Hp) eradication before and after eradication. Methods: The hTERT in gastric mucosa was detected by real-time quantitative fluorescent RT-PCR. 100 times of the ratio of copy number of hTERT to GAPDH (glyceraldehyde-3-phosphate dehydrogenase) was used as normalized hTERT (NhTERT) Hsp was detected by Warthin-Starry silver staining and p53 protein was detected by immunohistochemistry. Results: The expression rates of hTERT mRNA and p53 protein in Hp positive group were significantly higher than those in Hp negative subgroup (all P <0.05). The expression rate of hTERT mRNA in Hp eradication group was significantly lower than that in non-eradication group (P <0.05), but there was no significant difference between non-eradication group and negative control group. Conclusion: There is a direct relationship between Hp infection and telomerase expression and p53 protein in gastric precancerous lesions, suggesting that telomerase, Hp infection and p53 protein play an important role in the development of gastric carcinoma and the necessity of Hp eradication Sex.