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采用反相高效液相色谱法测定接骨丸中血竭素的含量 ,选用AlltechC18柱 ;乙腈 -0 .0 5mol/LNaH2 PO4 (4 2∶58)为流动相 ;检测波长为 4 40nm ;流速为 0 .7mL/min。该法平均回收率为 98.72 % ,相对标准差RSD为 1 .86(n =5)。该法快速、简便、灵敏度高 ,可作为该制剂的质控方法。
The contents of cathepsin in Jiegu Pills were determined by reversed-phase high performance liquid chromatography. Alltech C18 column was used. The mobile phase consisted of acetonitrile-0.05mol/L NaH2PO4 (42.58). The detection wavelength was 4 40nm. The flow rate was 0. .7mL/min. The average recovery of the method was 98.72%, and the relative standard deviation RSD was 1.86 (n = 5). The method is fast, simple and highly sensitive and can be used as a quality control method for the preparation.