目的　研究肝纤维化时Ⅳ型胶原酶（ＭＭＰ２）和金属蛋白酶组织抑制因子１（ＴＩＭＰ１） 在肝组织与贮脂细胞（ＦＳＣ） 中的表达，探讨其对肝纤维化的作用。方法　分别从正常和ＣＣｌ４ 肝纤维化大鼠的肝组织及ＦＳＣ中提取ＲＮＡ，应用逆转录聚合酶链反应（ＲＴＰＣＲ）方法检测ＭＭＰ２ 与ＴＩＭＰ１ ｍＲＮＡ 水平。结果　正常肝脏ＦＳＣ不表达ＭＭＰ２ ，仅肝纤维化时才转录；肝组织中无论是正常或肝纤维化时均存在ＭＭＰ２ ｍＲＮＡ，并在肝纤维化时表达增加，但无显著性差异（ Ｐ＞０ ．０５） 。ＴＩＭＰ１ 在正常与肝纤维大鼠的肝组织或ＦＳＣ中均表达，肝纤维化时肝组织和ＦＳＣ的ＴＩＭＰ１ ｍ ＲＮＡ 水平与正常相比显著升高（ Ｐ＜０ ．０５，Ｐ＜ ０．０２） 。结论　ＦＳＣ 表达ＭＭＰ２对肝纤维化发生具有重要意义，而ＴＩＭＰ１ 表达增加可能是导致肝纤维化过程中胶原降解减少的主要原因之一。 Objective To study the expression of type Ⅳ collagenase (MMP2) and tissue inhibitor of metalloproteinase1 (TIMP1) in liver tissue and fat-storing cells (FSC) during liver fibrosis and to explore its effect on hepatic fibrosis . Methods RNA was extracted from liver tissue and FSC of normal and CCl4 hepatic fibrosis rats, and the levels of MMP2 and TIMP1 mRNA were detected by reverse transcription-polymerase chain reaction (RTPCR). Results Normal liver FSC did not express MMP-2 and only translocated after liver fibrosis. MMP-2 mRNA was present in both normal and hepatic fibrosis in liver tissues, and increased in liver fibrosis with no significant difference (P> 0 .05). TIMP-1 in normal and liver fibrosis in rat liver or FSC were expressed in liver fibrosis liver tissue and FSC TIMP 1 m RNA levels were significantly higher than normal (P <0. 05, P < 0.02). Conclusion FSC expression of MMP  2 has important significance on the occurrence of liver fibrosis, and the increased expression of TIMP  1 may lead to reduced collagen degradation during liver fibrosis one of the main reasons.