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目的：研究脑缺血－再灌注损伤早期大鼠脑组织中ＩＬ－１β的表达及其与中性粒细胞浸润的关系。方法：应用反转录多聚酶链反应（ＲＴ－ＰＣＲ）检测ＩＬ－１βｍＲＮＡ，并用图象处理系统对正常、缺血及再灌注后不同时间的ＩＬ－１βｍＲＮＡ进行相对定量分析；采用ＭＴＴ和ＭＰＯ方法分别对大鼠脑组ＩＬ－１β的多肽活性及浸润的中性粒细胞进行检测。结果：单纯脑缺血ＩＬ－１β表达不增加；再灌注２ｈ，ＩＬ－１βｍＲＮＡ表达量明显增加，再灌注３ｈ，脑组织中ＩＬ－１多肽活性显著升高；再灌注９ｈ，中性粒细胞粘附到微血管壁并浸润到周围脑组织中。结论：脑缺血－再灌注损伤早期即有ＩＬ－１βｍＲＮＡ的表达并伴有相应活性多肽的合成分泌，与再灌注损伤后期的白细胞浸润密切相关。
Objective: To investigate the expression of IL-1β in brain tissue of rats with cerebral ischemia-reperfusion injury and its relationship with neutrophil infiltration. Methods: IL-1β mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR). Relative quantification of IL-1β mRNA in normal, ischemia and reperfusion groups was performed by image processing system. MTT and MPO The activity of IL-1β polypeptide and infiltration of neutrophils in rat brain were detected. Results: The expression of IL-1β in cerebral ischemia group was not increased. The expression of IL-1βmRNA increased obviously 2h after reperfusion, and the activity of IL-1 polypeptide in brain tissue increased remarkably 3h after reperfusion. At 9h after reperfusion, neutrophil adhesion Attach to the microvascular wall and infiltrate the surrounding brain tissue. CONCLUSION: The expression of IL-1βmRNA in the early stage of cerebral ischemia-reperfusion injury is associated with the synthesis and secretion of the corresponding active peptide, which is closely related to the leukocyte infiltration during the later stage of reperfusion injury.