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本文应用免疫细胞化学及ＮＳＥ－ＥＬＩＳＡ方法观察了一氧化氮对体外培养脊髓和背根神经节神经元的存活及对活性的影响。结果表明：一氧化氮合酶抑制剂Ｎ－Ａｒｇ组（１００、２００μｍｏｌ／Ｌ）ＮＳＥ免疫反应阳性神经元数目、面积（ＡＦ值）及活性（ＯＤ值）明显大于空白对照组（Ｐ＜０．０１）。而一氧化氮合酶底物Ｌ－Ａｒｇ组（１ｍｍｏｌ／Ｌ）神经元面积积分和活性则小于对照组（Ｐ＜０．０１）。Ｌ－Ａｒｇ的细胞毒性作用可为Ｎ－Ａｒｇ逆转。
The effects of nitric oxide on the survival and activity of neurons in spinal cord and dorsal root ganglion in vitro were observed by immunocytochemistry and NSE-ELISA. The results showed that the number, area (AF) and activity (OD) of NSE immunoreactive neurons in N-Arg group (100 and 200μmol / L) were significantly higher than those in blank control group (P <0. 01). However, the neuronal area integral and activity of nitric oxide synthase (L-Arg) group (1 mmol / L) were less than that of the control group (P <0.01). Cytotoxic effects of L-Arg can be reversed by N-Arg.